When using the tool for your experimnets, please cite:

Ulrich Krauss and Thorsten Eggert (2005) "insilico.mutagenesis: a primer selection tool designed for sequence scanning applications used in directed evolution experiments." BioTechniques 39(5): p679-682
 

This online tool allows the prediction of large sets of oligonucleotide primers used in saturation mutagensis and scanning mutagenesis experiments. Read the article mentioned above for more information. Abstract
The program generates the so-called mutagenesis primers to perform subsequent single amino acid exchanges within a defined region. Oligo's are designed in accordance with the MegaPrimer PCR approach as described by Barettino et al (1994).  For further information see the figure below. 
 
proceed to insilico.mutagenesis
 
The principle of the MegaPrimer PCR technique:
The online tool: insilico.mutagenesis will generate the in the figure highlighted mutagenesis primer which is used in the first PCR reaction of the megaprimer method.