Please provide the following information:
......vector size (in bp):

vector amount (in ng):

......insert size (in bp):

Please enter the molar vector : insert ratio:

(normally a vector to insert ratio of 1 to 3 is used of cohesive end ligations. higher molar ratios can be used for blunt end ligations)

When pressing the "do calculation" button the tool calculates the required amount of insert DNA (in ng) resulting in the given molar ratio